流式凋亡、周期檢測

2017-05-11

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實驗原理

CCK-8(Cell Counting kit-8)試劑中含有WST–8:化學(xué)名:2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二磺酸苯)-2H-四唑單鈉鹽], 它在電子載體1-甲氧基-5-甲基吩嗪硫酸二甲酯(1-Methoxy PMS)的作用下被細(xì)胞線粒體中的脫氫酶還原為具有高度水溶性的黃色甲臜產(chǎn)物(Formazan),生成的甲臜物的數(shù)量與活細(xì)胞的數(shù)量成正比,可采用酶聯(lián) 免疫檢測儀在450nm波長處測定其光吸收值,間接反映活細(xì)胞數(shù)量。

實驗流程

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MTT/CCK8檢測

500元

本價格包含實驗試劑盒,不包含細(xì)胞株及刺激藥物的購買